Description

CleanCap Reagent AU is designed for the co-transcriptional capping of mRNA to produce an mRNA with naturally occurring Cap 1. CleanCap Reagent AU was specifically designed for self-replicating RNAs based on the genomes of positive sense strand RNA viruses such as Venezuelan equine encephalitis virus (VEEV), Semliki forest virus (SFV) and Sindbis virus (SIN). The positive sense strand genomes of these viruses start with a 5'-AU... Cap 1 mRNAs have superior in vivo activity compared to Cap 0 mRNA produced by legacy cotranscriptional capping methods anti-reverse cap analog (ARCA). CleanCap can be used in conjunction with TriLink's catalog of modified NTPs.

Product Details

Catalog NoN-7114

Purity≥95% by AX-HPLC

Extinction Coefficient33,400 Lmol-1cm-1 at 258 nm

Molecular FormulaC31H42N12O25P4 (free acid)

Molecular Weight1106.6 g/mole (free acid)

Salt FormNa+

Concentration100 mM

BufferH2O

Recommended Storage-20°C or below

Other Name(s)CleanCap® AU for Self-Amplifying mRNA, CleanCap® Reagent AU for co-transcriptional capping of mRNA, m7G(5')ppp(5')(2'OMeA)pU

ApplicationCRISPR, In vitro Transcription, Recombinases, TALENS, Transposases, Vaccine Development, Zinc Finger Nucleases

Backbone5'-5'-Triphosphate

Base Analog(s)Adenosine, Uridine

Sugar Type(s)RNA

Nucleotide CategoryCap analogs